I created a segment that pulls a list of all users who do not have a specific custom property set in their profile, and then I programmatically update their properties from our database, including specifically setting that missing custom property.

However, when I then run the list again, those same profiles are still on the list.  I expected them to drop off of the list because I had set that custom property.

How do I refresh the segment so those profiles drop off and I can retrieve a new batch to process?