Segments in Klaviyo are designed to update automatically and in real-time. Once you create a segment, it will pull in customers that meet the criteria you set from anywhere in your Klaviyo account. This means that new profiles that match the segment criteria should be added automatically. How often do segments update? | Klaviyo Community
However, there are a couple of exceptions:
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Segments with relative time conditions: These segments update once every 24 hours. For example, if you have a segment for customers who made a purchase in the last 30 days, it will update daily to add or remove profiles based on their purchase activity. Understanding how segments update | Klaviyo Help Center
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Segments with negative conditions: These segments may experience slight delays in updating.
If you notice that new profiles are not being added to your segment as expected, it might be worth checking if your segment relies on relative time conditions or negative conditions